Thermo Fisher Scientific (Orbitrap Exploris120)

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공동활용서비스

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2021-12-29

화합물전처리/분석장비

경기도 성남시 수정구 성남대로 1342 가천대학교 B1층 B110호

- Vanquish UHPLC System- Thermo Scientific Hypersil GOLD VANQUISH C18 UHPLC Column / aQ UHPLC Column 100 x 2.1mm (Particle Sz.1.9) 보유- 표준 시료를 사용하여 LC-MS/MS 정성 및 정량 측정 가능- 생화학 물질(단백질, 지질, 대사체 등)의 고감도 동정(ID)법 구축 가능- Compound Discover 소프트웨어

*구성
1) High Resolution MS/MS system
- API source
- Quadrupole mass filter (Precursor isolation width from 0.4 Da to 2,000 Da or more)
- Flow rates : 1 ul/min ~ 1 ml/min using 100% water
2) U-HPLC System
- Binary Pump
- Autosampler
- Column Oven
3) Data System

*성능
1) Mass Range : Standard mass range m/z 40-3,000
2) Orbitrap mass analyzer Resolution : Up to 120,000 at m/z 200
3) Scan Rate : Up to 22 Hz at resolution setting 15,000 at m/z 200
4) Mass Accuracy
- External calibration achieves < 3 ppm RMS drift over 24 hours;
- Internal lock mass calibration achieves < 1 ppm RMS drift over 24 hours;
- EASY-IC achieves < 1ppm RMS drift for at least 5 days
5) Sensitivity
- MS/MS : 200 fg reserpine on column S/N 100:1
- tSIM : 200 fg reserpine on column S/N 250:1
6) Dynamic Range : > 5,000 within a single Orbitrap mass analyzer spectrum
7) Polarity Switching
- one Full Scan cycle < 700 ms equals > 1.4 Hz
- one tSIM Scan cycle < 600 ms equals > 1.6 Hz
8) Multiplexing : Up to 2 precursors per scan for tMS and up to 20 compounds per scan for tSIM
9) Analog Inputs : Channel 1 analog input (10 V), Channel 2 analog input (10 V)